How do corneal epithelial cells respond to a wound?

How do the initial signaling responses immediately after a wound translate into spatial coordinates for accurate wound healing?

How do the cells around a wound respond to the wound?

What are the sources of variability between cells?

What are the biological principles governing cellular variablilty?

Is cell variability a 'feature' or a 'bug'?

How are numerous cells segmented and tracked in time-lapse microscopy images?

What are the best methods to actually measure single-cell responses?

What technologies best enable us to measure multivariate gene expression responses?

What biological organizational principles allow intracellular and intercellular signaling networks to respond to a changing environment?